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Traditional elisa typically involves chromogenic reporters and substrates that produce some observable color change to indicate the presence of antigen or analyte It is a method of quantifying an antigen. Elisa is a laboratory technique that detects certain antibodies, antigens and other substances in your blood, pee or other bodily fluid

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Laboratory scientists use this technique for. Learn about the different methods for performing an elisa assay for protein quantitation, including assay design strategies and reagents.